东北山樱根际细菌群落结构多样性分析

Analysis of the Bacterial Community Structures Diversity in Rhizosphere of Cerasus sachalinensis Kom.

利用PCR-DGGE技术研究了野生与栽培两种生境下东北山樱的表层和根际土壤细菌群落结构的差异,以及野生东北山樱根际与其周围优势植物根际细菌群落结构的区别。用细菌的16S rDNA特异引物,对东北山樱和相邻其他优势植物根区土壤中提取的总DNA进行PCR扩增,通过DGGE技术对PCR产物进行分析。结果表明:无论在栽培条件下还是在野生条件下,东北山樱的根际土壤细菌群落结构多样性都高于表层土,且栽培条件下的多样性更高;野生东北山樱的根际土壤中形成特有的细菌群落,但其多样性低于对照和相邻的优势植物。

The bacterial community structures of Cerasus sachalinensis Kom. in topsoil and rhizospheric soil in wild and cultivated conditions were studied by means of PCR - DGGE （denaturing gradient gel electrophoresis）, and their differences in the rhizosphere of Cerasus sachalinensis and other neighboring plant in the pristine growing conditions were also analyzed. Partial genes were amplified from soil bacteri- al community DNA using 16S rDNA gene primers. The diversity of PCR-amplified products was detected by DGGE. The results show that the bacterial community diversities in Cerasus sachalinensis rhizospheric soil were always higher than in topsoil of both cultivated and wild conditions, and higher in the cultivated soil. In pristine growing conditions, the specific bacterial community structures were formed in Cerasus sachalinensis rhizosphere in the courses of plant growth and development. However, the rhizosphere bac- terial community diversities of Cerasus sachalinensis were higher than those of the other neighboring plant and the control.