氯丙嗪对大鼠皮层HVSs节律及面部抽搐增强作用研究

Chlorpromazine enhances high-voltage electroencephalogram spindles and facial twitching in freely moving rats

目的探讨氯丙嗪对正常成年大鼠自由活动状态下皮层HVSs节律以及面部抽搐的影响。方法通过在体多通道电生理记录技术结合视频录像，对可自由活动的正常大鼠进行皮层脑电和面部肌电长程记录，观察大鼠腹腔注射氯丙嗪后皮层HVSs节律个数、持续时间以及HVSs出现期间面部活动及肌电的变化。结果大鼠清醒静止状态下自发出现HVSs节律，面部偶发抽搐，腹腔注射低剂量氯丙嗪（1．5mg／kg）和高剂量氯丙嗪（3．0mg／kg）后均使HVSs节律个数增加．持续时间延长，大鼠面部肌肉节律性活动增多、持续时间延长。结论氯丙嗪能增强生理状态下出现的HVSs节律及面部抽搐，提示抗精神病药物锥体外系副作用可能与HVSs节律有关，而多巴胺受体可能参与大鼠皮层HVSs节律的调控。

Objective To explore the effect of chlorpromazine on neocortical high-voltage spindles （HVSs） and facial twitching in freely moving normal adult rats. Methods Continuous video, neocortical electroencephalogram （EEG） and facial electromyography （EMG） were recorded simultaneously for at least 6 h in freely moving rats. The number and mean duration of HVSs, and the facial muscular activity were analyzed aider administration of chlorpromazine. Results HVSs spontaneously appeared in behavioral waking-immobility rats with accidental hyperspasmia. After rats being administrated with chlorpromazine at a dose of 1.5 （mg/kg） and 3.0 （mg/kg）, the facial rhythmic muscular activity increased, the duration of rhythmic muscular activity prolonged, the number of HVSs increased and the mean duration of HVSs prolonged. Conclusion Chlorpromazine could enhance the physiological HVSs and facial twitching, indicating that the side effect at the extrapyramidal system of antipsychotic maybe relate to HVSs and dopamine receptor may contribute to the regulation of HVSs.