5-羟色胺2A受体T102C基因多态性与卒中后抑郁的关联研究

Association between polymorphism of 5-hydroxy tryptamine 2A receptor T102C gene and post- stroke depression

目的探讨5-羟色胺（5-HT）2A受体T102C基因多态性与卒中后抑郁（PSD）的关系，研究PSD的遗传发病机制。方法选择珠江医院神经内科自2010年1月至2010年12月收治的汉族住院患者为研究对象，根据有无并发抑郁症将其分为PSD组（97例）及单纯卒中组（72例）。采用PCR和限制性片断长度多态性（RFLP）技术测定2组患者5-HT2A受体T102C等位基因频率及基因型。结果PSD组中突变型C等位基因频率（43.3％）低于单纯脑卒中组（59．0％），突变纯合子C／C基因型频率（20．6％）也低于单纯卒中组（34．7％），差异均有统计学意义萨0．179，P=0．004；Х^2=7．855，P=-0．020）。结论5-HT2A受体T102C基因可能是PSD的易感基因，C等位基因是罹患PSD的保护因子。

Objective To explore the relationship between polymorphism of 5-hydroxy tryptamine 2A （5-HT2A） receptor T102C gene and post-stroke depression （PSD）, and investigate the heredity-related pathogenesis of PSD. Methods The polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） method was applied to test the allele frequencies and genotype of 5-HT2A receptor T102C gene in 97 patients with PSD and 72 patients without depression after stroke. Results The frequency of mutant C allele in patients with PSD was significantly lower than that in patients without depression （P〈0.05）, and the frequency of homozygote C/C genotype in patients with PSD was also obviously lower than that in patients without depression （P〈0.05）. Conclusion The 5-HT2A receptor T102C gene may be the susceptibility gene of PSD and C allele may be the protective factor of subjects suffering from PSD.