热化疗对荷瘤鼠及口腔癌患者CTL与HSP70表达水平影响的观察

Observation of impact of thermo-chemotherapy on the expression levels of cytotoxic T-lymphocyte and HSP70 in tumor-bearing mice and patients with oral cancer

目的:探讨热化疗对荷瘤鼠及口腔癌患者细胞毒性T淋巴细胞(CTL)及热休克蛋白70(HSP70)表达水平的影响,并初步探讨两者之间的相关性。方法:对Balb/c鼠进行热化疗处理,检测CTL活性及HSP70表达变化情况。20例口腔癌患者接受热化疗,于治疗前后行CTL活性检测,并行免疫组化检测HSP70表达情况。结果:热化疗组CTL活性与正常鼠差异无统计学意义(t=1.78,P=0.66),而与高温治疗组、平阳霉素治疗组和不治疗组比较差异有统计学意义,P=0.00。热化疗组HSP70表达明显增多,且FCM提示差异有统计学意义,P=0.00。临床试验中热化疗后口腔癌患者CTL及HSP70较热化疗前明显升高。结论:热化疗能显著提高荷瘤宿主CTL的活性,以及提高HSP70的表达,HSP70可诱导肿瘤细胞表达CTL。

OBJECTIVE： To investigate the impact of thermo-chemotherapy on the expression levels of cytotoxic T-lymphocyte（CTL） and heat shock protein（HSP70） in tumor-bearing mice and patients with oral cancer, and investigate the correlation between CTL and HSP70. METHODS： After treatments, the expression of CTL and HSP70 were detected, 20 cases of oral cancer patients received thermo-chemotherapy, the activity of CTL was detected before and after treatment, and the expression of HSP70 was detected on immunohistochemistry. RESULTS： CTL activity of thermo-chemotherapy group were no significant different from those of normal mice （t= 1.78,P=0. 66）, but they were significantly different from HS, CT and NT groups（P= 0. 00）. The expression of HSP70 on thermo-chemotherapy group were significantly increased, and the flow cytometry（FCM） suggested a significant difference from others （P=0. 00）. In clinical trials, the expression levels of CTL and HSP70 on oral cancer patients after thermo-ehemotherapy were significantly higher than those before thermo-chemotherapy. CONCLUSIONS： Thermochemotherapy can significantly improve the CTL activity of tumor-bearing hosts, and enhance the expression of heat shock protein 70. HSP70 can induce the expression of CTL in tumor cells.