微流控芯片技术在细胞生物学研究中的应用进展

Recent Applications of Microfluidic Technology in the Field of Cell Biology

20世纪90年代以来,微流控芯片技术得到了快速发展。由于具有小型化、集成化、高通量、低消耗、分析快速等特点,微流控芯片作为一种新型的生物学研究平台,能够提供传统方法不具备的精细和可控制的细胞研究条件,在细胞生物学研究领域中得到了广泛关注。该文主要介绍其在细胞培养、分选、裂解、计数、凋亡检测、迁移、单细胞捕获、细胞间作用等方面的研究进展。

Since the early 1990s, microfluidics, also called ＂lab on a chip＂ is growing rapidly. Because of having the characteristics of miniaturization, integration, high throughput, low energy consumption and rapid analysis, microfluidics becomes attractive in cell biological research as a promising platform. Microfluidic technology enables us to investigate cell behavior with precise and localized of experimental conditions which are unreachable by using macroscopic tools. This review mainly summarizes recent applications of microfluidics in cell culture, cell sorting, cell lysis, cell counting, cell apoptosis, cell migration, cell capturing and cell interaction.