摘要
应用ISSR分子标记技术对68份三角梅种质进行分析,从100个引物中筛选出14个多态性丰富、重复性好的引物,对68份三角梅种质资源的DNA多态性检测共获得了195条谱带,其中多态性条带182条,多态性比率为93.3%。聚类分析结果表明,三角梅品种间的遗传相似系数在0.50～0.97之间,平均相似系数为0.67,这说明供试的68份材料间的遗传多样性不是特别丰富、亲缘关系较近。在0.64水平处将三角梅种质资源分为A类群和B类群,A类群可分为2亚类,B类群还可分为6亚类。本研究从分子水平上揭示了三角梅不同种质资源之间的亲缘关系,进一步明确了种质资源间的遗传距离,为三角梅优良品质性状的选择、杂交育种亲本选配和良种繁育,提供了DNA分子水平上的理论依据。
Sixty-eight bougainvillea samples were analyzed using ISSR technique to study their taxonomic and phylogenetic relationships. Fourteen ISSR primers were used in the ISSR analysis of 68 bougainvillea individuals. A total of I95 ISSR bands were scored, corresponding to the polymorphic bands were 182, and the percentage of polymorphic was 93.3%. According to ISSR-PCR results, a cluster analysis （UPGMA） was used to generate a dendrogram among all 68 individuals. The results showed that genetic similarity among individuals was between 0.50 and 0.97, the average was 0.67. It was indicated that the relationship among all individuals was close and the genetic diversity was poor. According to dendrogram, 68 individuals could be divided into 2 groups at genetic similarity 0.64. The A group could be divided into 2 subgroups and B group could be divided into 6 subgroups. This study revealed the genetic relationship and confirmed the genetic distance among 68 bougainvillea individuals using molecular marker technology.
出处
《热带作物学报》
CSCD
2011年第9期1692-1696,共5页
Chinese Journal of Tropical Crops
基金
国家支撑计划:台湾农业新品种
新技术引进创新研究与示范(No.2007BAD07B00)子课题:菊花等闽台特色花卉新品种引进创新和离体保存研究(No.2007BAD07B03)
关键词
三角梅
ISSR
遗传多样性
亲缘关系
Bougainvillea
ISSR
Genetic diversity
Genetic relationship
