一种适用于多糖多酚植物的高质量RNA快速提取方法

Rapid Isolation of High-quality RNA from High Phenol-,Polysaccharide Plants

植物总RNA的提取是进行下游分子生物学实验的关键基础,高质量的RNA是下游实验成功的保障。许多植物由于其体内含有酚、多糖以及其他的一些次生代谢物,要获得这些植物高质量的总RNA比较困难。本文在多次实验的基础上,提出了改良LiCl沉淀法,该方法步骤少、效率高,使用此方法得到的棉花幼苗总RNA经检测表明其质量比使用冷酚法提取的RNA要高得多,且完全满足后续实验的要求。使用此方法成功提取了香蕉叶片、根、木薯叶片的RNA,经检测表明,所得到的RNA完整性好,完全可以满足后续实验的要求,说明改良LiCl沉淀法是一种适用于多酚多糖植物的快速RNA提取方法。

High-quality biologically active RNA isolation is crucialin plant molecular biological experiment. Many plants have high levels of endogenous phenolics, polysaccharides, and secondary metabolityes, so isolating high- quality biologically active RNA from these plants is very difficult. In this paper, a modified LiC1 RNA preparation method was developed to isolate RNA from cotton young plant, to yields high-quality total RNA. Then the high- quality RNAs were successfully isolated under this method from banana leaf, cassava leaf and banana root. All these results showed that the modified LiCI RNA preparation method is universal to isolate high-quality RNA from those plants that have high levels of endogenous phenolics and polysaccharides.