矮脚黄白菜核基因雄性不育系的创制与利用

Study on breeding and utilization of genetic male sterile line in dwarf Chinese cabbage

以复等位基因遗传的早熟大白菜雄性不育"两用系"AB01和"临时保持系"B01为试材,设计定向转育方案,采用连续回交转育性状的同时,测交筛选基因型的方法,向源自金冠的矮脚黄白菜自交系Y中转育雄性不育基因,育成了不育株率和不育度均为100%、园艺学性状与自交系Y相近的矮脚黄白菜核基因雄性不育系GMS401。结果表明,用GMS401试配的杂交组合(GMS401×D1)和(GMS401×D2)整齐度高,园艺学性状优异,产量显著高于对照品种南京矮脚黄和抗病矮脚黄。

A directional transfer program was designed by using multiple allele inherited genetic mate-sterile line AB01 and its temporary maintainer line B0, as source of male sterility, and recurrent backcrossing was employed to transfer botanical traits, while the genotype was identified through test cross. Following the procedure, the male sterile gene was successfully transferred to the target parent ＂Y＂, the inbred line of Jinguan in dwarf Chinese cabbage. A new male sterile line GMS,01, which is similar to Y on botanical traits with 100% male sterility and 100% male sterile plants, was bred. The results showed that GMS4ol as the female parent, the hybridized combinations GMS401 x D1 and GMS401 x D2 were finally obtained and they were excellent in horticultural characters and uniformity. In addition, the yield of the combinations was apparently higher than that of CK.