QSA的mRNA在人和小鼠各组织中表达规律

Distribution and Expression of Gene QSA mRNA in Different Tissues of Human and Mouse

从基因水平上检测精子中新基因QSA的表达规律及时空分布,并推测其可能的生物学作用。采用RT-PCR和原位杂交法检测QSA在人和小鼠多种组织中的表达。RT-PCR结果显示QSA在小鼠的睾丸、精子和受精卵中特异性表达,而在小鼠卵细胞、囊胚等组织中未检测到该基因的mRNA。原位杂交结果表明QSA在人的部分腺体组织(如:睾丸、甲状旁腺等)中显著表达。根据基因QSA表达模式,推测其mRNA在获能精子中被选择性保留,可能与某种激素的产生或分泌相关,并且可能参与精子发生或胚胎早期发育过程,从而为精子传递具有父系效应的mRNA提供新的证据。

To detect the expression pattern of QSA at gene level,and speculate the possible biological function of QSA,RT-PCR and in situ hybridization（ISH） were performed to detect the expression of QSA mRNA in different tissues of human and mouse.QSA transcripts were demonstrated in mice testis,ejaculated sperm and zygote,but undetectable in mice ovum,blastocyst and other tissues.ISH results showed significantly high level of QSA mRNA in human testis and a number of glandular tissues.Due to QSA＇s expression pattern,we speculate that QSA mRNA is selectively retained in capacitated sperm,and it may be related to the production or secretion of a hormone.This implies that QSA mRNA is possibly involved in spermatogenesis or early embryo development.The result provides new evidence that human sperm can deliver mRNA transcripts that is provided with paternal effects.