吐温80与氢氧化钠耦合污泥溶胞及生物水解酸化特性研究

Research on Characteristic of Biological Hydrolysis and Acidification of Excess Sludge after Lysis by Combining Tween80 and Sodium Hydroxide

采用吐温80与氢氧化钠耦合进行污泥溶胞,当污泥溶胞时间为15min时,污泥上清液中蛋白质与DNA浓度较初始上清液中分别提高了32.5倍和15.8倍,显示细胞壁已得到有效破解,胞内的蛋白质与DNA溶出进入水相。而且,溶胞后污泥的水解酸化过程中挥发性脂肪酸(VFAs)和氨氮产量随着吐温80浓度的增大和酸化时间的延长而增加,这表明吐温80的加入促进了水解酸化菌对蛋白质和碳水化合物的分解。酸化进行24h时,不同条件下系统的pH均达到7.4左右,此后系统进入稳定运行阶段。

TweenSO coupled with sodium hydroxide was used to promote the effect of sludge lysis. When the sludge lysis time was 15 min, the concentrations of protein and DNA in the supernatant were respectively 32.5 and 15.8 times higher than those in the initial supernatant. This indicated that the cell wall had been cracked and the intracellular protein and DNA had been extracted into the supernatant. Moreover, the concentrations of VFAs and NH3-N increased with the increase of the concentration of Tween80 and time during the acidification of the sludge after lysis. The results showed that the addition of Tween80 could contribute significantly to the hydrolyzing effect of proteins and carbohydrates by acidification bacteria. After 24 h of acidification, the pH under different conditions was close to 7.4, and then the system went into a stable operation.