利培酮与氯氮平对离体胰岛细胞凋亡的影响

Effect of risperidone and clozapine on rat islet cells apoptosis in vitro

目的:探讨利培酮和氯氮平对体外培养胰岛细胞凋亡的影响。方法:利用细胞培养技术,在一定的葡萄糖浓度(5.5 mmol/L)和不同作用时间(1 h或4 h)下,以1μmol/L利培酮和氯氮平分别作用于体外培养大鼠胰岛细胞,以单染法流式细胞仪检测作用后胰岛细胞的凋亡率,并与相应的对照组比较。结果:利培酮作用于胰岛细胞1 h和4 h后,细胞凋亡情况与对照组比较,差异无统计学意义(P>0.05);氯氮平作用于胰岛细胞1 h和4 h后,细胞凋亡情况与对照组比较,差异有统计学意义(P<0.05);氯氮平作用于胰岛细胞4 h后与作用1h后相比,细胞凋亡差异无统计学意义(P>0.05)。结论:氯氮平可以诱导离体大鼠胰岛细胞凋亡,导致胰岛细胞DNA含量减少,而利培酮在本研究中引起离体胰岛细胞发生凋亡的现象不明显。

Objective：To explore the effects of risperidone and clozapine on the apoptosis of rat islet cells in vitro.Methods：Using cell culture technique,under certain glucose concentration（5.5 mmol/L） and different action times（1 h or 4 h）,1 umol/L risperidone and clozapine were acted on the rat islet cells cultured in vitro respectively.The apoptosis rate of islet cells after action was detected by single staining flow cytometry and compared with the corresponding control group.Results：After 1 h and 4 h of risperidone acting on islet cells,apoptosis occurred,but compared with the control group,there was no statistical difference（P0.05）.After 1 h and 4 h of clozapine acting on islet cells,apoptosis occurred,which showing statistical significance as compared to the control groups（P0.05）.Comparing after 1 h of clozapine acting on islet cells with after 4 h,there was no statistical significance（P0.05）.Conclusion：Clozapine can induce the apoptosis of rat＇s islet cells cultured in vitro,result in the decrease of the quantity of DNA in islet cells.Risperidone,on the contrary,does not cause the apoptosis of islet cells cultured in vitro in this research.