乙型肝炎病毒感染Bewo细胞模型的建立

Construction of Bewo cell model with hepatitis B virus infection

目的:建立乙型肝炎病毒(HBV)感染人胎盘滋养层细胞系Bewo的细胞模型,为HBV母婴传播过程中胎盘细胞感染机制的研究提供体外细胞模型。方法:选择人胎盘滋养层细胞系Bewo进行体外培养,用高浓度HBV DNA阳性血清(5×106/拷贝.ml-1)与Bewo细胞共培养48 h后,采用选择性PCR技术检测培养细胞中HBVrcDNA和HBVcccDNA。结果:HBV DNA阳性血清与Bewo共培养48 h,Bewo细胞中HBV rcDNA和HBV cccDNA均呈现阳性结果(243 bp和373 bp)。HBVDNA阳性血清与Bewo共培养对细胞形态无明显影响。结论:HBV能够直接感染人胎盘滋养层细胞系Bewo,该细胞系可用于进行HBV母婴传播过程中胎盘细胞感染机制的研究。

Objective：To construct a model of human placental trophoblast Bewo cell lines infected by hepatitis B virus（HBV）,provide an extracorporeal cell model for researching the mechanism of placental cell infection during the course of HBV maternal-neonatal transmission.Methods：Human placental trophoblast Bewo cell line was selected for culture in vitro,Bewo cells were cocultured with high dose positive HBV DNA sera（5×106/copy·ml-1） for 48 hours.Selective PCR technique was used to detect HBVrcDNA and HBVcccDNA in Bewo culture supernatant.Results：After 48 hours,positive HBVrcDNA and HBVcccDNA were found in Bewo cells（243 bp and 373 bp）.Coculture of positive HBV DNA sera and Bewo cells had no obvious impact on cell morphology.Conclusion：HBV can infect human placental trophoblast Bewo cell line directly,which can be used to study the mechanism of placental cell infection during the course of HBV maternal-neonatal transmission.