硅胶塞玻璃试管压力蒸汽灭菌冷凝水干燥方法的研究

STUDY OF CONDENSATE DRYING METHOD FOR RUBBER PLUG TUBES BY PRESSURE STEAM STERILIZATION

目的观察胶塞试管压力蒸汽灭菌冷凝水残留的相关因素及其干燥方法。方法通过改进胶塞试管摆放方法和分段干燥措施,解决其干燥的问题。结果采用4个干燥时段,到第4时段所有倒置摆放的胶塞试管全部干燥,且明显比传统侧放的胶塞试管干燥快,两组差异有非常显著统计学意义(P<0.01)。随干燥时间递增,两组干燥合格率均随之上升,倒置摆放在每个时段干燥速度均高于侧放组。倒置摆放组试管内化学监测和生物监测全部合格,灭菌后试管无菌培养全部无菌生长。结论胶塞试管在压力蒸汽灭菌器内常规侧放很难干燥,采用倒立装放并延长干燥时间能提高干燥速度,且不影响灭菌效果和无破损现象。

Objective To observe the relevant factors of remain condensate from pressure steam sterilization on rubber plug tubes and its drying methods.Methods Changing display manner and applying sub-drying method were used to resolve the drying problem.Results The whole time was disparted to four subsections.Until the forth subsection,all inverse-placed rubber plug tubes had dried which were quicker than traditional method obviously.The difference was significant on statistics（P0.01）.Qualified rate increased with drying time prolonged,inverse-placed tubes dried quicker than side-placed tubes in each subsection.All inverse-placed tubes were qualified in chemical test and biological test.Results of bacterial culturing were negative as well.Conclusion Side-placed rubber plug tubes are difficult to dry in pressure steam sterilizer traditionally.Adopting inverse-placed method and prolong drying time can enhance drying speed based on sterilization qualified and non-broken.