丁香乙醇提取液对临床分离菌株的抗菌作用及毒性试验

STUDY ON ANTIMICROBIAL EFFICACY AND TOXICITY OF CLOVE ETHANOL EXTRACT ON CLINICALLY ISOLATED MICROORGANISM STRAINS

目的研究丁香乙醇提取液的体外抗菌作用和毒性。方法采用抑菌试验和动物试验方法,观察了丁香乙醇提取液对结核分枝杆菌、蜡状芽孢杆菌、肺炎克雷伯菌等临床分离菌株和大肠杆菌、金黄色葡萄球菌等标准菌株的抑菌环直径均大于7 mm。在40 mg/ml的浓度下对细胞无毒性伤害。小鼠急性毒理试验LD50值大于5 000mg/kg,长期毒理试验LD50值大于5 000 mg/kg。结论丁香乙醇提取液具有良好的体外抗临床菌株作用,毒性低,具有开发成消毒液的前景。

Objective To investigate the antimicrobial activity and toxicity of the ethanol extract from clove.Methods Antibacterial method and animal test were used to observe the antimicrobial activity of the ethanol extract from clove.Results The diameters of bacterial inhibition zones of clinically isolated microorganism strains including Mycobacterium tuberculosis,Bacillus subtilis and Klebsiella pneumoniae,and standard microorganism strains including Escherichia coli and Staphylococcus aureus exposed to the clove ethanol extract were 7 mm.The mouse acute oral LD50 was 5 000 mg/kg,the mouse long-time toxicity LD50 was 5 000 mg/kg.Conclusion The clove ethanol extract has good clinically antimicrobial efficacy and low toxicity.It can be developed as a disinfectant in the future.