组织芯片技术研究P16^(INK4A)和Ki67在宫颈鳞状上皮病变中的表达

Expressions of P16INK4A and Ki67 in cervical squamous intraepithelial lesions by tissue microarray

目的:利用组织芯片技术及免疫组化技术检测在正常宫颈组织、宫颈上皮内瘤变及宫颈鳞状细胞癌中P16INK4A和Ki67的表达情况及其意义。方法:应用免疫组化技术检测53例宫颈组织(正常宫颈9例,宫颈上皮内瘤变32例,宫颈鳞状细胞癌12例)制成的组织芯片中P16INK4A和Ki67的表达,并比较3组的表达情况及其相互关系。结果:P16INK4A和Ki67在正常组中阳性率分别为0.00%(0/9)和11.11%(1/9);CIN组中阳性率分别为78.13%(25/32)和68.75%(22/32);鳞状细胞癌组中阳性率均为91.67%(11/12)。其中正常组与CIN组、正常组与癌组、正常组与CIN组和癌组比较差异极有统计学意义(P<0.01)。此外,P16INK4A和Ki67的表达密切相关(P<0.05)。结论:P16INK4A和Ki67表达与宫颈鳞状上皮病变的发生发展密切相关,两者表达相关性提示可能共同参与肿瘤的发生发展。

Objective：To investigate the expressions and significance of P16INK4A and Ki67 in normal cervical tissue,cervical intraepithelial neoplasia（CIN） and cervical squamous cell carcinoma by tissue microarray technology and immunohitochemistry techenique.Methods：The immunohitochemistry technique was adopted to detect the expressions of P16INK4A and Ki67 of tissue microarray in 53 cases of cervical tissue（9 cases of normal cervix,32 cases of CIN and 12 cases of cervical squamous cell carcinoma）.The expressions in the three groups were compared their correlation was analyzed.Results：The positive rates of P16INK4A and Ki67 were 0.00%（0/9） and 11.11%（1/9） in the normal group,78.13%（25/32） and 68.75%（22/32） in the CIN group and both 91.67%（11/12） in the squamous cell carcinoma group.The difference between the normal group and the CIN group,between the normal group and the cancer group,and between the normal group with CIN group and the cancer group showed statistical significance（P0.01）.In addition,P16INK4A expression is closely related with Ki67 expression（P0.05）.Conclusion：The expression of P16INK4A and Ki67 is closely related to the occurrence and development of cervical squamous intraepithelial lesions,the correlation between their expressions prompts that they maybe jointly participate in the occurrence and development of tumor.