不同切制法对楤木根皮中皂苷类成分的影响

Influence of Saponins from Root Bark of Aralia chinesis by Different Cutting Method

目的:研究不同切制法对楤木根皮中皂苷类成分的影响。方法:采用鲜切法和干切法对楤木根皮进行切制,以属性较强的显色法进行总皂苷含量测定,并以HPLC测定皂苷元齐墩果酸的含量。结果:齐墩果酸在2.08～10.4 mg.L-1线性关系良好(r=0.999 5),平均回收率98.33%,RSD 0.83%。鲜切与传统切制根皮总皂苷平均质量分数分别为8.26%,6.59%;鲜切与传统切制根皮齐墩果酸质量分数分别为5.26%,5.23%。结论:楤木根皮采用鲜切法优于传统切制法,能有效降低皂苷类成分的损失,为今后将楤木开发应用提供参考。

Objective：To study on effect of saponins from root bark of Aralia chinesis with different cutting methods.Method： Using fresh cutting method and dry cutting method to cut root bark of A.chinesis,determinated total saponins by spectrophotometry which is a specificity for saponins.At the same time,determinated the content of oleanolic acid by HPLC.Result：Regression equations revealed good linear relationships between peak-area ratios and concentrations.Oleanolic acid had good linearity in the range of 2.08-10.4 mg·L-1（r=0.999 5）,average recovery was 98.33 %,RSD 0.83 %（n=6）.The content of total sapnins from root bark of A.Chinesis with fresh-cut method was 8.26%,dry cutting was 6.59%.The content of oleanolic acid was 5.26 % and 5.23 % respectively.Conclusion： Root bark of A.chinesis using fresh-cut method was superior to conventional dry cutting method,it could effectively reduce losses of saponins component,it could supply reference for development and utilization of A.chinesis in future.