摘要
目的:建立桔梗高效液相色谱-蒸发光散射检测器(HPLC-ELSD)指纹图谱的分析方法,研究不同产地桔梗药材的质量。方法:采用HPLC-ELSD,Welch Materials XB-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%甲酸溶液,梯度洗脱,流速0.8 mL.min-1,柱温30℃,ELSD检测器漂移管温度50℃,氮气流速1.2 L.min-1。结果:建立了12批桔梗的HPLC-ELSD指纹图谱,共找到16个共有峰,并确定了桔梗皂苷E,桔梗皂苷D3和桔梗皂苷D。结论:该方法具有良好的重复性和稳定性,为有效控制桔梗药材的质量提供了依据。
Objective: To establish a method of fingerprint analysis for Platycodonis Radix by high performance liquid chromatography-evaporative light-scattering detector(HPLC-ELSD).Method: HPLC was performed on a Welch Materials XB-C18 analytical column(4.6 mm × 250 mm,5 μm) with gradient elution of acetonitrile-0.1% formic acid at the flow rate of 0.8 mL·min-1 with ELSD detector.The column temperature was maintained at 30 ℃,the temperature of drift tube was set at 50 ℃,and the gas flow(N2) was set at 1.2 L·min-1.Result: The HPLC-ELSD chromatographic fingerprint of chemical constituents was established from the 12 batches of Platycodonis Radix and it had 16 characteristic common peaks.Conclusion: The developed method is repeatable and stable,which is helpful to control the quality of Platycodonis Radix.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第22期50-53,共4页
Chinese Journal of Experimental Traditional Medical Formulae