HPLC测定知母-黄芪药对中芒果苷、毛蕊异黄酮苷和毛蕊异黄酮

Simultaneous Determination of Mangiferin,Calycosin-7-O-β-D-glucoside and Calycosin in Anemarrhenae Rhizoma-Astragali Radix by HPLC

目的:建立知母-黄芪中芒果苷、毛蕊异黄酮苷和毛蕊异黄酮3种有效成分的含量测定方法。方法:采用HPLC,Phenomenex Luna C18(4.8 mm×250 mm,5μm)色谱柱,加预柱(Security Guard,C18,3.0 mm×4.0 mm,Phenomenex);以乙腈-0.2%磷酸溶液为流动相,梯度洗脱,流速1.0 mL.min-1,柱温30℃,检测波长260 nm。结果:芒果苷、毛蕊异黄酮苷和毛蕊异黄酮的线性范围分别为36.733～550.999 ng,33.376～166.88 ng,44.544～222.72 ng,相关系数(R2)均>0.999,平均加样回收率分别为102.47%,102.38%,100.20%,其RSD分别为2.23%,2.86%,2.06%。结论:方法简便快速,重复性良好,结果准确可靠,可用于知母-黄芪中芒果苷、毛蕊异黄酮苷和毛蕊异黄酮的含量测定。

Objective： A reversed-phase high performance liquid chromatographic method（RP-HPLC） was developed for the simultaneous analysis of mangiferin,calycosin-7-O-β-D-glucoside and calycosin in Anemarrhenae Rhizoma-Astragali Radix.Method：HPLC determination was performed on a Phenomenex Luna C18（4.8 mm×250 mm,5 μm） and detected at 250 nm.The mobile phase was consisted of acetonitrile and 0.2% phosphate solution with gradient elution.Flow-rate was 1.0 mL·min-1,and column temperature was 30 ℃.Result： The method was proved to be linear in the ranges of 36.733-550.999 ng,33.376-166.88 ng and 44.544-222.72 ng for mangiferin calycosin-7-O-β-D-glucoside and calycosin respectively.The average recoveries were 102.47%,102.38% and 100.20% respectively.Conclusion： The results showed that the method is convenient,accurate and reliable for the simultaneous determination of mangiferin,calycosin-7-O-β-D-glucoside and calycosin in Anemarrhenae Rhizoma-Astragali Radix.