双参宁心方的正常与心肌缺血模型大鼠血清对缺氧/复氧H9C2细胞作用的比较

Comparison of Effects of Shuangshen Ningxin Serum Obtained from Normal Rat and Myocardial Ischemia Rat on H/R H9C2 Myocardial Cells

目的:比较大鼠在正常状态与心肌缺血状态下制备的双参宁心方(SSNX)空白及含药血清对体外缺氧/复氧H9C2心肌细胞活力的影响。方法:分别设立正常组、心肌缺血模型组。大鼠心肌缺血模型由冠状动脉结扎法建立,术后缝合伤口继续喂养。术后2 h除正常空白、模型空白、模型假手术组给予生理盐水外,均按高、中、低剂量(180,90,45 mg.kg-1)分别ig给予SSNX 5日。末次给药后30,90,150 min分批处死大鼠,分别制备正常及模型血清。将其作用于缺氧/复氧H9C2细胞。MTT法检测H9C2细胞活力。结果:正常空白血清与模型空白血清对缺氧/复氧H9C2心肌细胞活力影响差异明显(P<0.05),正常动物SSNX含药血清与心肌缺血模型动物SSNX含药血清均有提高缺氧/复氧H9C2心肌细胞活力的作用,分别与正常动物空白血清及模型动物血清比较(P<0.05);各时间点正常含药血清与相应时间点模型含药血清相比更显著提高了H9C2心肌细胞活力。结论:2种方法制备的SSNX血清对体外缺氧/复氧H9C2心肌细胞活力的作用趋势一致,但强度有一定差异。

Objective：To compare the effects of pathological model Shuangshen Ningxin（SSNX）serum with normal SSNX serum on hypoxia /reoxygenation（H/R） injury H9C2 cell citality.Method：Model rat of myocardial ischemia injury was established by coronary arterial ligating.Anterior descending branch of coronary artery was ligated.Except for normal blank,model blank,sham operation group,Normal rats and model rats are consecutively gavaged with SSNX at high,medium and low doses 2 h after the operation for 5 days consecutively.Collect blood from abdominal aorta 30,90 and 150 min after last drug administration,and prepare serum.Construct H9C2 myocardial cell H/R injury model.Respectively administrate serums containing drugs from normal rat and model rat to H/R H9C2 cells.Determine the cell citality by using MTT method.Result： There is a difference between normal blank serum group to model blank serum group in cell vitality（P〈0.05）.Compared to normal blank and model blank group,cell activity SSNX serum group of normal and myocardium ischemia rat model are obviously different（P〈0.05）;and the effects of SSNX serum group of normal is better than group of model at different time points（P〈0.05）.Conclusion： The effects of SSNX serum prepared by two ways,is analogous on the role in the trend,but is different on degree.