黄芪不同有效部位配伍对骨髓抑制模型小鼠粒系调控因子的影响

Effect of Compatibility of Astragali Radix Effective Parts on Granulocytopoiesis in Myelosuppression Mice

目的:考察黄芪不同有效部位配伍对骨髓抑制模型小鼠粒细胞-巨噬细胞集落刺激因子(GM-CSF)、粒细胞集落刺激因子(G-CSF)、肿瘤坏死因子-β(TNF-β)的影响。方法:BALB/c小鼠实验第1,3,5天ip环磷酰胺100 mg.kg-1复制骨髓抑制模型。分为正常组、模型组、黄芪饮片组、酮苷组、糖苷组、糖酮组、糖苷酮组。造模当日给药,正常组、模型组按10 mL.kg-1ig蒸馏水;黄芪饮片组按10 g.kg-1 ig黄芪水煎液;各有效部位配伍组按黄芪生药量10 g.kg-1分别ig相应治疗药物(酮苷组0.227 g.kg-1,糖苷组0.610 g.kg-1,糖酮组0.514 g.kg-1,糖苷酮组0.678 g.kg-1)。连续ig 15 d。ELISA法检测小鼠骨髓细胞GM-CSF,G-CSF,TNF-β含量。结果:糖苷组、糖酮组GM-CSF含量明显增加(P<0.05);糖苷组、糖酮组G-CSF含量明显增加,且优于黄芪饮片组(P<0.05);黄芪不同有效部位配伍组TNF-β含量均明显减少,且糖苷组减少TNF-β含量优于酮苷组、糖酮组、糖苷酮组(P<0.05)。结论:黄芪有效部位可能通过调控细胞因子调控粒系造血;黄芪多糖与皂苷、黄酮配伍后促进粒系造血效果较优。

Objective：To sduty the effect of compatibility of Astragali Radix effective parts on granulocyte-macrophage colony-stimulating factor（GM-CSF）,granulocyte colony-stimulating factor（G-CSF）,tumor necrosis factor-β（TNF-β） in myelosuppression mice.Method：BALB/c mice were given cyclophosphamide intraperitoneally at 100 mg·kg-1 on the first,third,fifth day of the experiment to built myelosuppression mice model.The mice were divided into uninfluenced group,model group,Astragali Radix group,ketoside group,glucoside group,saccharon group,glucoside and ketone group.At the same time mice in uninfluenced group and model group were given water ig with the dosage of 10 mL·kg-1.Mice in Astragali Radix group were given the decoction ig with the dosage of 10 mL·kg-1.Mice in groups of compatibility of Astragali Radix effective parts were given corresponding medicine（ig） with the dosage of Astragali Radix 10 g·kg-1 for 15 days： ketoside group 0.227 g·kg-1,glucoside group 0.610 g·kg-1,saccharon group0.514 g·kg-1,glucoside and ketone 0.678 g·kg-1.Detected GM-CSF,G-CSF,TNF-β,levels in bone marrow cells with ELISA.Result：GM-CSF in glucoside group,saccharon group increased significantly（P0.05）.G-CSF in glucoside group,saccharon group increased significantly and were higher than Astragali Radix group（P0.05）.TNF-β in Groups of compatibility of Astragali Radix decreased significantly and glucoside group was lower than ketoside group,saccharon group,glucoside and ketone（P0.05）.Conclusion：Compatibility of Astragali Radix effective parts may regulate granulocytopoiesis through the regulation of cell factors.Astragali Radix polysaccharides with Astragali Radix flavone or astragaloside accelerated granulocytopoiesis significantly.