线叶菊抗菌作用研究

Studies on Antibacterial Activity of Filifolium sibiricum

目的:研究线叶菊体内、外的抗菌作用,并对其作用机制进行初步探讨。方法:体外抗菌实验采用微量稀释法测定线叶菊对12种临床常见病原菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。体内抗菌实验采用小鼠ip细菌的干酵母混悬液,观察7 d内动物的死亡率。利用透射电镜对部分病原菌与线叶菊作用前后的细菌形态学进行考察。结果:线叶菊对12种临床常见病原菌有不同程度的抑菌和杀菌作用,特别是对耐药金黄色葡萄球菌的MIC为0.43 g.L-1,MBC为0.86 g.L-1。体内实验高剂量组对由金黄色葡萄球菌、大肠杆菌、绿脓杆菌、耐药金黄色葡萄球菌和耐药肺炎克雷伯氏菌导致的感染动物均具有保护作用,呈量效关系,中剂量组对绿脓杆菌和耐药金黄色葡萄球菌仍有作用。电镜结果显示线叶菊能够消融细菌的细胞壁。结论:线叶菊对12种临床常见病原菌的体外抗菌作用均优于双黄连口服液,特别是对头孢阳性对照组无作用的耐药绿脓杆菌仍具有抑菌和杀菌作用。体内抗菌实验结果表明线叶菊对小鼠全身感染动物模型具有保护作用,特别对头孢克肟无作用的耐药金黄色葡萄球菌导致的感染动物依然具有保护作用。同时线叶菊能够破坏细菌的细胞壁。

Objective：To study the antibacterial effect of Filifolium sibiricum（FS）and its possible mechanism preliminarily.Method： Vitro experiments using micro dilution method to determine minimum inhibitory concentration（MIC） and minimum bactericidal concentration（MBC） of 12 species common clinical pathogens.The anti-microbial activity of FS was observed mice mortality within 7 days by injecting dry yeast suspension of bacteria in intraperitoneal.Antibacterial mechanism was investigated by transmission electron microscope.Result：FS had different levels of bactericidal effects to 12 species common clinical pathogens.Especially for drug-resistant Staphylococcus aureus,MIC is 0.43 g·L-1 and MBC is 0.86 g·L-1.In vivo high dose can protect animals infected by S.aureus,E.coli,Pseudomonas aeruginosa,drug resistant S.aureus and drug resistant Klebsiella pneumoniae.The dose group was still effect to P.aeruginosa and drug resistant S.aureus.Electron microscopy showed that FS can melt bacterial cell wall.Conclusion：FS antibacterial activity was superior than Shuanghuanglian oral solution in vitro to 12 clinical common pathogens,especially to drug resistant P.aeruginosa which the positive control group had no role.The vivo experiments results show that it has a protective effect to systemic infectied animal model,especially to drug resistant S.aureus infections still has a protective effect.It can destroy the cell walls of bacteria at the same time.