家蝇幼虫乙醇提取物的抗炎解热作用及机制

Effect and Mechanism on Anti-inflammatory and Antipyretic of Ethanol Extracts from Musca domestica

目的:研究家蝇幼虫乙醇提取物(MDE)对酵母致炎发热大鼠的解热作用及机制。方法:利用干酵母致炎大鼠发热动物模型,设立生理盐水组:ig等体积生理盐水;模型组:ig等体积生理盐水+大鼠背部sc 15%干酵母20 mL.kg-1;阿司匹林组:ig阿司匹林0.3 g.kg-1;给药组:分别ig MDE(1,0.5,0.25 g.kg-1),qd,预防性连续给药7 d(n=10),观察MDE对发热大鼠直肠温度的影响,测定给药后不同时间的体温变化,并采用放免法测定下丘脑中前列腺素E2(PGE2)和环磷腺苷(cAMP)含量。结果:1,0.5,0.25 g.kg-1MDE具有不同程度的退热作用;并能减少致热大鼠下丘脑中PGE2及cAMP的含量,高剂量组(126.86±24.89)ng.g-1,(9.72±1.11)nmol.g-1;中剂量组(180.12±34.58)ng.g-1,(10.05±0.26)nmol.g-1;低剂量组(203.54±30.46)ng.g-1,(10.79±0.57)nmol.g-1;与模型组(253.70±43.96)ng.g-1,(12.15±3.11)nmol.g-1比较具有显著性差异(P<0.01,P<0.05)。结论:MDE具有解热作用,其作用机制与降低下丘脑中PGE2以及cAMP有关。

Objective：To study the anti-inflammatory and antipyretic effect and mechanism of the ethanol extracts from Musca domestica larvae（MDE） on febrile rats.Method：The rats fever model were made by dry yeast.Saline group was given equal volumes saline（ig）;Model group was given equal volumes saline（ig）＋ sc 15% dry yeast 20 mL·kg-1 of subcutaneous injection;Treatment group： MDE（1,0.5,0.25 g·kg-1）,ig qd for 7 d,Rectum temperature（ΔT/ ℃）was measured and hypothalamus proataglandin E2（PGE2） and adenosine cyclophosphate（cAMP） were detected by RIA.Result：1,0.5,0.25 g·kg-1MDE had antipyretic effect on febrile rats and could reduce the content of PGE2 and cAMP ·kg-1）（126.86±24.89） ng·g-1,（9.72±1.11） nmol·g-1],·kg-1）（180.12±34.58） ng·g-1,（10.05±0.26） nmol·g-1],·kg-1）（203.54±30.46） ng·g-1,（10.79±0.57） nmol·g-1],campared to the control（253.70±43.96） ng·g-1,（12.15±3.11） nmol·g-1）（P〈0.01,P〈0.05）.Conclusion：The results indicate that ethanol extracts from M.domestica larvae has anti-inflammatory and antipyretic effect,the mechanism may be related to reducing PGE2 and cAMP.