三桠乌药叶片中挥发油成分及抑菌活性研究

GC-MS Analysis of Essential Oils from Leaves of Lindera obtusiloba

目的:研究三桠乌药叶片中所含挥发油成分及抑菌活性。方法:采用水蒸气蒸馏法提取三桠乌药叶片中所含挥发油,并用气相色谱-质谱联用(GC-MS)技术,分析挥发油的化学成分。用滤纸片固相扩散法和平板连续稀释法进行挥发油抗菌活性试验和最小抑菌浓度(MIC)研究。结果:首次提取了三桠乌药叶片中所含挥发油,成功鉴定出46种化合物,并对其抑菌活性进行了研究,发现其具有广谱抗菌性。结论:三桠乌药叶片中所含挥发油主要成分为醇类化合物、萜类化合物和少量氧化物、酯类化合物及其他物质。其对供试菌的抑菌强度由大到小为金黄葡萄球菌>表皮葡萄球菌>粪肠球菌>大肠埃希菌>桔青霉>白色葡萄球菌>枯草芽孢杆菌>白色念球菌>黑曲霉>绿脓杆菌。

Objective： To study the components of the essential oil from the leaves of Lindera obtusiloba and the antimicrobial intensity.Method： Distillation in water vapor was applied to extract the essential oil from the leaves of L.obtusiloba and meanwhile gas chromatography-mass spectrometry（GC-MS） was used to analyze the components of this plant.Meanwhile,paper disc diffusion method and plate serial dilution method were used respectively to study the anti-bacterial activity and the minimum inhibitory concentration（MIC）.Result： The essential oil was extracted successfully and 46 compounds were identified.Also broad-spectrum antimicrobial activity was found.Conclusion： Main components of the essential oil from the leaves of L.obtusiloba are alcohol,sesquiterpenes,and a minority of esters,oxygenated components as well as some other compounds.And the antimicrobial intensity to the tested strains is： Staphylococcus aureus Staphylococcus epidermidis Enterococcus faecium Escherichia coil Penicillium citrinum Staphylococcus albus Bacillus subtilis Candida albicansAspergillus niger Pseudornonas aeruginosa.