清络通痹颗粒含药血清对破骨细胞骨吸收的影响

Effects of Qingluo Tongbi Granule-containing Serum on Bone Resorption by Osteoclasts

目的:确定清络通痹颗粒(QLT)含药血清的制备方法并观察其对体外培养破骨细胞的活性和骨吸收的影响。方法:选用高效液相色谱外标一点法测定,QLT含药血清中青藤碱的含量以确定含药血清的制备条件;乳鼠破骨细胞1×108/mL加入96孔板,将QLT 3.6,7.2,14.4 g.kg-1给药的大鼠含药血清1∶10稀释,分别加入各孔培养48 h,观察其对破骨细胞增殖、抗酒石酸酸性磷酸酶(TRAP)活性及破骨细胞形成的骨吸收陷窝的影响。结果:QLT 7.2,14.4 g.kg-1给药的含药血清可明显抑制破骨细胞的增殖能力和吸收陷窝面积(P<0.05,P<0.01),QLT 3.6,7.2,14.4 g.kg-1给药的含药血清可明显抑制破骨细胞的TRAP活性(P<0.05,P<0.01)。结论:QLT含药血清可抑制破骨细胞增殖、TRAP活性和骨吸收能力,对破骨细胞引起的骨质破坏产生抑制作用。

Objective： To observe effects of Qingluo Tongbi granule-containing serum on bone resorption of osteoclasts in vitro.Method： The content of sinomenine in Qingluo Tongbi granule-containing serum was detected by HPLC in different time point to decide the method for obtaining the drug-containing serum.The osteoclasts from newborn rats were cultured and the proliferation,TRAP activity and bone resorption were detected.Result： The rats were given the drugs for 7 days and the drug-containing serum was obtained one hour after the last administration.The serum could inhibit the proliferation,TRAP activity and bone resorption area of osteoclasts.Conclusion： Qingluo Tongbi granule-containing serum could restore the bone erosion by inhibiting proliferation,TRAP activity and bone resorption ability.