蠲痛饮对大鼠子宫内膜异位症微血管密度的影响

Effect of Juantongyin on Microvessel Density of the Experimental Endometriosis in Rats

目的:探讨蠲痛饮对大鼠子宫内膜异位症(endometriosis,EMs)微血管密度(microvessel density,MVD)的影响和抗血管形成作用。方法:采用自体子宫内膜移植法建立EMS大鼠模型,将60只大鼠分为正常组、模型组、蠲痛饮低、中、高剂量组、丹那唑组6组,分别给予蠲痛饮高、中、低剂量(42.9,14.3,4.8 g.kg-1)和丹那唑(0.021 5 g.kg-1)ig,模型组及空白组按照10 mL.kg-1给予生理盐水ig,持续用药28 d,SP免疫组化法检测各组EMS大鼠在位内膜和异位组织灶中MVD的表达。结果:丹那唑组和高、中、低剂量蠲痛饮减小大鼠异位组织体积分别为(32.96±15.38),(15.75±8.36),(38.16±17.32),(42.91±19.54)mm3,与模型组(89.23±26.27)mm3比较差异有统计学意义(P<0.05)。丹那唑组和高、中、低剂量蠲痛饮降低EMS大鼠异位内膜MVD的计数(18.61±5.89,12.24±4.82,14.36±4.13,16.53±4.56),与模型组(26.43±6.75)比较差异有统计学意义(P<0.05)。结论:蠲痛饮对子宫内膜异位症大鼠异位组织中MVD的表达和新生血管形成均有抑制作用,作用呈剂量依赖性。

Objective： To observe the effect of Juantongyin（JTY） on microvessel density（MVD） and antiangiogenesis in ectopic endometrium of rats.Method： To observe the effect of high-dose,middle-dose,low-dose JTY and danazol on MVD of endometriotic rats through setting up endometriosis model with rat self-endometria transplantation.Sixty rats were divided into 6 groups randomly：JTY high-dose group（42.9 g·kg-1）,middle-dose group（14.3 g·kg-1）,low-dose group（4.8 g·kg-1）,and danazol group（0.021 5 g·kg-1）,model group and control group（10 mL·kg-1） of saline.Result： There was a marked diversity among model group（89.23±26.27） mm3 and other groups（32.96±15.38）,（15.75±8.36）,（38.16±17.32）,（42.91±19.54） mm3（P〈0.05） of the ectopic tissue volume.There was a marked diversity among model group（26.43±6.75）,and other groups（18.61±5.89,12.24±4.82,14.36±4.13,16.53±4.56）,（P〈0.05） of the expression of MVD.Conclusion： JTY has inhibitory effect on the expression of MVD and angiogenesis in ectopic endometrium of rats,in a dose-dependent manner.JTY may be a new drug of antiangiogenesis in ectopic endometrium.