毛鸡骨草醇提液对HepG2.2.15细胞乙型肝炎表面抗原及乙型肝炎E抗原的影响

Effect of Abrus mollis Hance on HBsAg and HBeAg in the HepG2.2.15 Cells

目的:观察毛鸡骨草体外抗乙型肝炎病毒(HBV)的作用。方法:采用MTT法检测毛鸡骨草对HepG2.2.15细胞的半数毒性浓度(TC50)和最大无毒浓度(TC0),采用酶联免疫吸附实验(ELISA)法测定<TC0的5个不同浓度的毛鸡骨草醇提液对HepG2.2.15细胞株表达的乙型肝炎表面抗原(HBsAg)和乙型肝炎E抗原(HBeAg)的影响。结果:毛鸡骨草醇提取液可有效地抑制细胞HBsAg和HBeAg的分泌;在质量浓度为4 g.L-1作用144 h对HBsAg,HBeAg抑制作用最为明显,抑制率分别为33.1%,40.1%。结论:毛鸡骨草在体外有一定的抗HBV作用。

Objective： To observe the inhibitive effect of Abrus mollis Hance on HBV.Method：The TC50 and TC0 of A.mollis to the HepG2.2.15 ceils were determined by MTT assay.The effect of TC0 five different concentrations of alcohol extract of A.mollis on HBsAg and HBeAg in the HepG2.2.15 cells was determined by ELISA.Result： A.mollis had a inhibiting effect on the HBsAg and HBeAg with maximum inhibition ratio adding up to 33.1% and 40.1%,respectively,in which the affective time was 144 h and the density was 4 g·L-1.Conclusion： A.mollis can significantly inhibit HBV in vitro.