气相色谱-质谱联用法测定卷烟主流烟气气相物中挥发性有机化合物

Determination of Volatile Organic Compounds in Gas-phase Mainstream Smoke by Gas Chromatography-mass Spectrometry

建立了气相色谱-质谱联用（GC-MS）同时快速检测卷烟主流烟气中5种挥发性有机化合物（1,3-丁二烯,异戊二烯,丙烯腈,苯和甲苯）的分析方法。主流烟气气相物中的挥发性有机化合物被含有D6-苯内标的冷甲醇吸收,并用GC-MS在选择离子模式下进行分析。方法的最低检出限（LOD）和最低定量限（LOQ）分别为0.01~0.81μg/支和0.04~2.73μg/支,加标回收率为92.3%~98.5%,精密度为1.95%~9.21%。本方法检测限低,重复性和重现性明显优于以前报道的方法。应用此方法考察了38种国内外卷烟样品在深度抽吸与ISO抽吸模式下的释放量,深度抽吸模式下挥发性有机化合物的释放量是ISO模式的两倍。

A gas chromatography-mass spectrometry（GC-MS） method was successfully developed and validated for simultaneous determination of five Volatile Origanic Compounds（VOC： 1,3-butadiene,isoprene,acrylonitrile,benzene and toluene） in cigarette gas-phase mainstream smoke.Before smoking,each impinger was spiked with D6-benzene internal standard solution.The gas-phase mainstream smoke was trapped by two impingers filled with methanol cool solution.And then,five VOCs were analyzed by GC-MS under selected ions recording mode.The limit of detection and limit of quantification for each VOC were varied from 0.01 to 0.81 μg/cig and 0.04 to 2.73 μg/cig.Recoveries were in the ranged of 92.3% to 98.5% and intra-and inter-assay precision（RSD%） were 1.95 to 9.21.This new VOCs determination method provided higher sensitivity,specificity,and repeatability than earlier methods.Five VOCs levels of 38 commercially available domestic and overseas cigarettes were determined under ISO（International Organization for Standards） and Canadian intense smoking regime by the proposed method.VOCs levels in mainstream smoke under Canada intense smoking regime were twice higher than ISO regime.