烤烟品种幼苗低温反应敏感度与其生化差异性研究

The Difference of Biochemistry and the Sensitivity to Low Temperature in Flue-Cured Tobacco Varieties

以烤烟品种中烟100、NC82、K326为材料,于幼苗五叶一心期,分别进行2℃、5℃、8℃低温处理,通过对不同处理下幼苗的相对电导率、POD、丙二醛和脯氨酸等生化指标分析,对不同品种间低温反应敏感度进行研究。结果表明,3个品种幼苗的相对电导率、MDA、POD、脯氨酸均随低温胁迫强度的增加呈增大的趋势;3个品种均在2℃和5℃表现较强的低温敏感性,其中2℃条件下培养3 d最敏感,8℃处理7 d最不敏感。

Flue-cured tobacco varieties with different chilling tolerance,such as Zhongyan100,NC82,K326,were treated with 2 ℃,5 ℃and 8 ℃ at 5th-leaf stage.The sensitivity to low temperatures of flue-cured tobacco varieties were investigated according to the biochemical indexes,such as relative electrical conductivity,MDA,Peroxidase activity,Proline content and so on.The results demonstrated that,relative electrical conductivity,Peroxidase activity,MDA content and Proline content all obviously rise under the low temperature stress;In all the three tobacco varieties,the low temperature sensitivities are higher under 2 ℃ and 5 ℃,and the most under 2 ℃ treatment of 3 days,the least under 8 ℃ treatment of 7 days.