摘要
目的探讨环加氧酶(COX)-2抑制剂尼美舒利(NIM)对人舌鳞状细胞癌Tca8113细胞中ang-2基因表达的影响及其意义。方法以25、50、100和200μmol.L-1浓度的NIM处理Tca8113细胞48 h,半定量RT-PCR检测其ang-2 mRNA的表达。结果 Tca8113细胞中ang-2 mRNA水平下降,且下降呈浓度依赖性。结论 NIM下调ang-2基因表达以抑制肿瘤血管发生,可能是COX-2抑制剂抑瘤的作用机制之一。
Objective To investigate the influence of Nimesulide(NIM),a selective cyclo-oxygenase(COX)-2 inhibitor,on angiogenins(ang) gene expressions in human tongue squamous cell carcinoma Tca8113.Methods Tca8113 cells were treated with NIM(25,50,100,200 μmol·L-1) for 48 h to meaure the expression of ang-2 mRNA in Tca8113 cells by the semiquantitative reverse transcription polymerase chain reaction analysis.Results The expression of ang-2 mRNA reduced in a concentration dependent manner.Conclusion NIM can down-regulate ang-2 gene expression,which may be involved in cancer angiogenesis inhibition mechanisms of COX-2 inhibitor.
出处
《国际口腔医学杂志》
CAS
2011年第6期646-648,共3页
International Journal of Stomatology
基金
山西省科技攻关计划基金资助项目(200703109-5)
关键词
舌鳞状细胞癌
环加氧酶
血管发生
血管生成素
tongue squamous cell carcinoma
cyclo-oxygenase
angiogenesis
angiogenin