神经节苷脂对弥散性脑损伤模型大鼠认知功能的改善作用研究

Improvement Effect of Ganglioside on Cognitive Function of Rats after Diffuse Brain Injury

目的:研究神经节苷脂对弥散性脑损伤模型大鼠认知功能的改善作用。方法:取大鼠48只,随机分为正常对照组、假手术组、模型组和药物干预组(神经节苷脂40mg·kg-1·d-1),每组12只,后2组均按Marmarou方法建立弥散性脑损伤模型,建模后30min各组经腹腔注射相应药物;给药14d后,采用Morris水迷宫试验和跳台试验比较各组大鼠的认知功能。结果:与正常对照组和假手术组比较,模型组和药物干预组大鼠搜索安全平台次数明显减少,在靶像限活动时间、跳台潜伏期均明显缩短,第1次穿过平台时间、跳台错误次数明显增加(P<0.05);与模型组比较,药物干预组上述指标均发生改善(P<0.05)。结论:弥散性脑损伤对大鼠认知能力有一定影响,神经节苷脂可改善弥散性脑损伤模型大鼠的认知障碍。

OBJECTIVE： To investigate the improvement effect of ganglioside on cognitive function of rats after diffuse brain injury. METHODS： 48 rats were randomly divided into normal control group, sham operation group, model group and drug intervention group （ganglioside 40 mg.kg-1.d -1） with each group of 12 rats. The diffuse brain injury model of model group and drug intervention group were established by Marmarou method. 30 min after modeling, each group was given intraperitoneally corresponding drugs. After 14 days, Morris water maze and step-down test were used to compare cognitive functions among those groups. RESULTS ： Compared with normal control group and sham operation group, the times of cross safety island in model group and drug intervention group decreased significantly. The time of targeting orthant and incubation period decreased significantly, while the time of crossing island at the first time and the number of step-down errors increased significantly （P〈0.05）. Compared with model group, above indexes of drug intervention group were all improved （P〈0.05）. CONCLUSION： Diffuse brain injury may influence the cognitive function of rats. Ganglioside is effective in the recovery of cognitive ability.