Smad4和转化生长因子-β_1在肝细胞癌组织芯片中的表达和意义

Expressions of Smad4 and transforming growth factor-β_1 in human hepatocellular carcinoma tissue array and its significance

目的研究Smad4和转化生长因子-β(1TGF-β1)在肝细胞癌组织芯片中的表达和意义。方法使用组织芯片技术,选取200例肝细胞癌患者癌组织及癌旁肝组织,32例肝血管瘤患者瘤旁正常肝组织进行对照,分别进行Smad4和TGF-β1免疫组织化学染色,在显微镜下观察其表达的阳性率。结果 Smad4在对照组肝组织、癌旁肝组织、肝细胞癌中的表达呈逐渐下降的趋势,即Smad4在癌组织中表达有缺失,差异有统计学意义。在表达强度上,癌旁肝组织以强阳性表达为主,肝细胞癌中主要以弱阳性表达为主。TGF-β1在癌旁肝组织中的阳性表达率为96%,高于对照组,但在肝细胞癌中的阳性表达率降低为49.5%。对照组肝组织中的TGF-β1表达以弱阳性为主,占40.6%,在癌旁肝组织中以中度阳性为主,占51.5%,在肝细胞癌组,以阴性表达为主,占50.5%。由对照组到癌旁组织到肝细胞癌组,TGF-β1的表达呈现先升后降的趋势。结论 Smad4、TGF-β1与肝细胞癌的发生和肿瘤的生物学行为密切相关,TGF-β1可能作为肿瘤抑制因子和促进因子,发挥双向调节作用,缺失导致肝细胞癌发生,但过高表达可能使肿瘤细胞侵袭能力加强。

[Objective] To investigate the expression of Smad4 and TGF-β1 in human hepatocellular car- cinoma （HCC） tissue array and its significance. [Methods] Tissue array and immunohistochemical staining were used to detect the expression of Smad4 and TGF-β1 in 200 cases of HCC, adjacent noncancer liver tissues （ANLT） and 32 normal controls, then observed and recorded the positive rate by microscope. [Results] The expressions of Smad4 in normal controls, ANLT and HCC were decline, that is, the expression of Smad4 was lost in HCC. And there were strongly positive in ANLT, but weakly in HCC. It was 96% cases expressed TGF-β in ANLT, which was higher than normal controls, but was significantly low in HCC. The weakly positive expressions of TGF-β was mainly in normal controls, it was 40.6%, and in ANLT the main expressions were moderate positive, it was 51.5%, and there were 50.5% cases negative expression in HCC. It was up af- ter the first from normal controls to ANLT and HCC of the TGF-β1 expression. [Conclusion] The expressions of Smad 4 and TGF-β1 may play an important role in the occurrence and progression of HCC.