酸王苹果被迫休眠期的呼吸代谢特征

Characteristics of respiratory metabolism during Avrolles' imposed dormancy

以萌发极晚的苹果品种酸王(Malus domestic cv.Avrolles)和对照品种瑞林(M.domestic cv.Judeline)为试材,测定被迫休眠期的呼吸速率、呼吸途径改变及呼吸途径关键酶活性的变化来研究酸王被迫休眠期的呼吸代谢特征,为选育避开晚霜冻害的晚萌发品种提供生理依据。结果表明,尽管酸王和瑞林芽内呼吸途径变化趋势一致,但运行活性和时间进程有明显差异。酸王的总呼吸和EMP途径呼吸强度低于瑞林,最高点分别是瑞林的84.6%和84.7%,PPP途径增加的时间比瑞林长21 d,且运行活性高于瑞林,变化幅度是瑞林的2.02倍;呼吸途径各关键酶活性、呼吸底物含量均低于瑞林,这些是酸王被迫休眠期的呼吸代谢方面的特征,在被迫休眠期的呼吸代谢提供的能量不充足限制了酸王的萌发。

In order to provide physiological bases for selecting late-germination cultivars that can avoid late frost damage, the very late-germination cultivar Malus domestic cv. Avrolles and the control cultivar M. domestic cv. Judeline were used as experimental materials to investigate the characteristics of the respiratory metabolism, the changes of respiratory pathway, respiratory rate and the key enzyme activity during imposed dormancy. The results showed that： changes of respiratory pathway in buds of Avrolles and Judeline had the same trend, but the running activity and time processing of the change were significantly different between the two varieties. The total and EMP respiration intensity of Avrolles were less than that of Judeline, the peak value was 84.6% and 84.7% respectively. Time for PPP accumulation in Avrolles was 21 days longer than that in Judeline, the running activity was higher and the amplitude was 2.02 times as that in Judeline. The activity of key enzymes and content of respiratory substrates in Avrolles were lower than that in Judeline. The energy provided by respiratory metabolism was not sufficient, which limited the ~ermination of Avrolles.