重组ALA合酶基因(YHem1)转化草莓提高叶片光合能量转化能力的效应

Effect of transformed recombinant ALA synthase gene(YHem1) on leaf photosynthetic energy conversion in strawberry

为了培育高光合抗逆草莓新品种,以红颊草莓试管苗叶片为外植体,研究了植物生长调节剂对草莓叶片再生的影响,然后将拟南芥HemA1基因启动子控制的酿酒酵母ALA合酶基因(Hem1)的重组基因(YHem1)通过农杆菌介导法转入草莓离体叶片中。经卡那霉素筛选,PCR和RT-PCR检测,证明外源基因已经转入草莓体内,获得3株转基因植株。叶片快速叶绿素荧光特性测定显示,转基因草莓叶片PSII最大光化学效率(Fv/Fm)、光合性能指数(PIABS)和捕获的激子将电子传递到QA-下游的其他电子受体的概率(ψo)显著高于野生型,而QA被还原的最大速率(Mo)则低于野生型,说明转入YHem1基因提高了草莓叶片光合能量转化能力。

In order to breed new varieties with higher photosynthetic capacity under stress conditions, the experiments were carried out to establish regeneration system from leaf explants of Benihonppe strawberry （Fragaria ananassa Duch.） and then a recombinant gene YHeml, yeast ALA synthase gene controlled by Arabidopsis thaliana HemAl gene promoter, was transformed into the detached leaves mediated with A- grobacterium tumefaciens. After kanamycin selection, PCR and RT-PCR detection, three plants were i- dentified to be the transgenic plants. The chlorophyll fast fluorescence showed that the transgenic straw- berry remained higher leaf maximal photochemical efficiency （F、/Fm）, photosynthetic performance index （PI_ABS） and probability of a trapped exciton moving an electron into the electron transport chain beyond QA- （ψ.）, while the approximate initial slope of the fluorescence transient （Mo） was lower in transgenie plants than in the wild type. This suggested that Yheml transformation might increase the capacity of photosyn- thetic energy conversion in strawberry leaves.