基于液相色谱-质谱联用技术的代谢组学方法用于中药通心络和人参对过度疲劳大鼠干预作用的评价

Evaluation of the effect of the traditional Chinese medicine Tongxinluo or ginseng on excess fatigue rats studied by metabonomics approach based on liquid chromatography-mass spectrometry

用代谢组学方法评价了中药通心络和人参对过度疲劳大鼠的干预作用。通过构造大鼠过度疲劳模型,并分别用通心络和人参进行干预,采用快速液相色谱-离子阱飞行时间质谱(UFLC-IT-TOF-MS)获取大鼠血浆代谢轮廓,并用正交偏最小二乘法(OPLS)进行多变量统计分析,分别找出用于区分通心络和人参干预组大鼠同正常对照大鼠、过度疲劳大鼠的重要差异代谢物。结果显示过度疲劳大鼠体内的色氨酸、胆汁酸、溶血磷脂酰胆碱等代谢通路发生较大变化,经通心络或人参干预的大鼠整体代谢轮廓趋向正常水平,并能够部分调节上述发生变化的代谢通路使之往正常方向变化。

Excess fatigue is a pathological state of continuing accumulation of fatigue,which may cause the deterioration of body health,occurrence of diseases,and even lead to death.A metabonomics study was performed on the excess fatigue rats treated with traditional Chinese medicine Tongxinluo or ginseng based on ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry（UFLC-IT-TOF-MS）.The plasma metabolic profiling data of the control rats,excess fatigue rats,and excess fatigue rats treated with Tongxinluo or ginseng were acquired.The orthogonal partial least squares analysis（OPLS） was applied for the multivariate statistics and the discovery of important differential metabolites distinguishing the excess fatigue rats treated with Tongxinluo or ginseng from the control rats and excess fatigue rats.The results showed tryptophan,bile acid,lysophosphatidylcholine metabolism were disturbed in the excess fatigue rats.The metabolic pattern including the related metabolic pathways of the rats,being treated with Tongxinluo or ginseng,was adjusted towards the normal state.