Intermedin预处理大鼠肾脏缺血再灌注损伤修复过程中CyclinE和CDK2表达的变化

The change of CyclinE and CDK2＇s expression in Intermedin pretreatment in rat renal ischemia- reperfusion injury and repair

目的观察Intermedin（IMD）预处理大鼠肾脏缺血再灌注损伤（IRI）修复过程中细胞周期篮向E（eyclinE）和其依赖性激酶2（CDK2）表达的变化，IMD可能的促进肾组织冉生修复的作用机制。方法健康雄性Wisiar大鼠，体重180—220g，共144只随机分为对照组、IRI组、转空质粒组、转IMD组。对照组和IRI组切除右肾；转空质粒组、转IMD组大鼠切除右肾后，在六氟化硫微泡（声诺维）介导下将空质粒及IMD质粒转染入左肾；l周后分别制作肾脏IRI模型。每组于再灌注ld、2d、3d、4d、7d、14d时各取6只留取肾组织标本。检测各组肾组织中cyclinE与CDK2的表达。结果IRI绀cyclinE、CDK2于再灌注后ld、2d、3tl、4rl、7d表达逐渐增高，7d时到达最高峰，此后表达逐渐降低，14d时降至最低，但仍有少量表达，与对照组比较差异具有统计学意义（P〈0．05）。转IMD组各项指标在第ld即开始最著增高，第2d、3d、4d、7d呈进行。陀下降，至14d时恢复正常，与IRI组相比其差异具有统计学意义（P〈0．05）；转空质粒组与IRI组以。卜指标差异无统计学意义。结论IMD预处理大鼠肾脏缺血再灌注损伤后早期，cyclinE、CDK2的表达明显上调，提示MD可能通过促进细胞周期进展从而加快肾组织再生修复。

Objectives lntermedin （IMD） pretreatment in rat renal ischemia- reperfusion injury （IRI） during the repair process cyclin E （cyclinE） and its dependent kinase 2 （ CDK2 ） expression changes, IMD may promote kidney tissue regeneration and repair mechanisms. Methods Male Wistar rats weighing 180-220g, 144 were randomly divided into control group, IRI group, empty vector transfected group, turn IMD group. Control group and the removal of the right kidney IRI group; turn empty plasmid group, turn right kidney removed after the IMD group, the sulfur hexafluoride microbubbles （SonoVue） mediated plasmid and empty plasmid transfected into the left renal IMD ; were made after 1 week of renal IRI model. Each group in the reperfusion ld, 2d, 3d, 4d, 7d, 14d from each of six specimens from the time of renal tissues. Renal tissue were detected by cyclinE and CDK2 expression. Results IRI group cyclin E, CDK2 after repeffusion Id, 2d, 3d, 4d, 7d expression gradually increased, 7d reach the peak time, then gradually decreased expression, 14d when the minimum, but there is still a small amount of expression, compared with the control group the difference was significant （ p 〈 0. 05 ）. IMD Group switch indicators began in ld significantly increased on 2d, 3d, 4d, 7d decreased progressively, from 14d to resume normal, compared with the IRI and the differences were statistically significant （ p 〈 0. 05 ） ; empty vector transfected group and the IRI group no significant difference between the above indicators. Conclusions IMD pretroatment in rat kidney after isehemia and reperfusion injury early, cyclinE, CDK2 expression was significantly increased, suggesting that IMD could promote cell cycle progression through to speed up the regeneration of kidney tissue.