儿童特发性血小板减少性紫癜患者NK细胞及T淋巴细胞亚群变化及意义

Study on changes and clinical significance of lymphocyte subtypes and NK cells in children with idiopathic thrombocytopenic purpura

目的检测特发性血小板减少性紫癜(ITP)患者T淋巴细胞亚群及NK细胞的变化,探讨其在ITP发病机制中的作用。方法应用流式细胞术分别检测80例ITP及35例正常对照组T淋巴细胞亚群、NK细胞变化。结果 ITP患者CD3+、CD4+细胞百分比及CD4+/CD8+比值明显低于正常对照组(P<0.01),CD8+细胞百分比高于正常对照组(P<0.01)。NK细胞百分比明显低于正常对照组P<0.01)。结论及T淋巴细胞亚群及NK细胞的变化可较好的反映ITP的病理过程,对提高ITP的诊断水平及指导临床具有一定的使用价值。

Objective To test changes in T lymphocyte subsets and NK cells in patients with ITP and to discuss the related factors envolved in pathlg nalysis detect T lymphocyte and NK cells subtype enesis of ITP. Methods Using flow cytometric a- in 80 patients with ITP and 35 heaithy controls. Resoits The percentage of CD3^＋ T lymphocyte and CD4^＋ T lymphocyte and the ratio of CD4^＋/CD4^＋ in patients with ITP were obviously lower than those in healthy controls （P 〈0. 01 ） ,but the percentage of CD8^＋ T lymphocyte was much higher （P 〈 0. 01 ）, the percentage of NK cells was significantly lower than that in heaithy controls （P 〈 0. 01 ）. Conclusions The changes in T lymphocyte and NK ceils subsets can reflect the pathogenesis of ITP well. What is more, the test of them has a certain practical value in improving the diagnosis of ITP and guiding clinic.