慢性乙型肝炎血清HBV DNA基因含量与临床的相关性研究

Relationship between clinics and content of serum HBV DNA in chronic hepatitis B

目的探讨慢性乙型肝炎(慢乙肝)患者病毒复制水平与HBV标志物(HBV-M)模式及肝损害程度的关系。方法 360例慢性乙型肝炎(轻度160例、中度140例、重度60例)患者血清HBV DNA含量采用荧光标记(AmpliSensor)定量PCR方法检测,HBV-M检测采用ELISA法。结果血清HBV DNA含量与HBV-M模式有关,血清HBeAg阳性组HBV DNA含量(106.35±1.84)显著高于HBeAg阴性组(104.73±1.88)(P<0.01),慢乙肝轻度、中度患者HBV DNA含量[(105.58±1.92),(106.27±2.05)]与慢乙肝重度患者HBV DNA含量(105.73±1.90)相比较无显著性差异(P>0.05),且不同HBV DNA水平患者的TBil、ALT、AST差别无显著性(P>0.05)。结论血清HBeAg的存在影响HBV DNA的水平变化,随着肝损害程度的加重,慢性乙型肝炎轻度、中度、重度患者血清HBV DNA基因含量未发生显著变化,同时血清HBV DNA含量与TBil、ALT、AST水平无明显关系。

Objective To explore the relationship between the nucleic acid content of hepatitis B virus and hepatitis B virus markers, liver function and its pathological state of patients with different clinical types of chronic hepatitis B. Methods Fluorescence quantitative PCR was used to measure the HBV DNA and ELISA was used to detect the hepatitis B virus markers in 360 cases. Results Serum HBV DNA content was related to hepatitis B virus markers. The HBV DNA content in the group of HBeAg positive（ 106. 35± 1.84）was significantly higher than that of HBeAg negative（ 104.73 ± 1.88） （P 〈0.01 ）. There were not significant difference between HBV DNA content and different clinical types of chronic hepatitis B （P 〉 0. 05 ）. Conclusions There was a significant correlation between HBeAg and HBV DNA,but not between liver inflammation stage and serum HBV DNA content in dif- ferent clinical types of chronic of hepatitis B.