用肝肾共培养体系研究细胞毒性的方法学考察

Methodological Study on Cell Toxicity with Liver and Kidney Co-Culture System

目的建立肝肾共培养体系,并证明此体系的合理性与可行性方法采用MTT法比较川楝素(20、40、80、160μg/mL)、葛根素(200、100、50、25μg/mL)、苦参碱(5、2.5、1.25、0.625 mg/mL)、汉防己乙素(50、25、12.5、6.25 mg/mL)在肝肾单独培养和共培养体系中对肝、肾细胞活力的影响。结果 2种体系中:川楝素仅对肝细胞的活力有抑制作用,葛根素对肝肾细胞均无抑制作用,苦参碱对肝细胞活力的抑制程度大于肾细胞,汉防己乙素对肾细胞活力的抑制程度大于肝细胞。结论用肝肾细胞共培养体系与单独培养体系进行实验得出的结果一致,这2种体系均可用于药物对细胞的毒性研究,其中共培养体系能更好地反映药物对靶器官的选择性。

Objective To establish the liver and kidney co-culture system and prove its rationality and feasibility. Methods The effects of Toosendanin （20, 40, 80, 160 ptg/mL）, puerarin （200, 100, 50, 25 lag/mL）, matrine （5, 2.5, 1.25, 0.625 mg/mL） and hanfangchin b （50, 25, 12.5 6.25 mg/mL） on the liver and kidney cell activity in the liver and kidney separate culture and co-culture systems were compared with MTT. Results In two systems, Toosendanin only inhibited the activity of liver cells, puerarin had no effect on both liver and kidney cell, the inhibition of matrine on liver cell viability was greater than kidney cells, while kidney cells were restrained more serious by hanfangchin b. Conclusion Experimental results were consistent in the two systems, which both can be used to study the toxicity of drugs on cells. Co-culture system responds better to the target organ selectivity.