酶联免疫反应加速仪在检测丙肝抗体中的应用

The Roles of Enzyme-linked Immunosorbent Accelerometer Played in Detecting Antibody to Hepatitis C Virus

目的探讨酶联免疫反应加速仪在酶联免疫吸附试验（ELISA）检测丙型肝炎抗体中的应用。方法用酶联免疫反应加速仪和常规温育方法同步检测4种不同S／CO的混合血清，同时用实时荧光定量聚合酶链反应法检测临界值水平血清／灰区标本的HCV．RNA，并对结果进行比较分析。结果4种混合血清用两种方法检测的抗-HCV吸光度值（OD值）比较，差异有显著性（P〈0．05）。有3种混合血清定性结果相一致，S／CO为0．9的混合血清用常规温育法检测结果为阴性，用酶联免舨应加速仪检测结果为阳性，而检测20份S／CO为0．9的血清的HCV—RNA含量，其中14份大于1×10^3拷贝／mL。4种混合血清，酶联免疫反应加速仪的OD值CV％分别为2．5、2．0、2．1、2．3，常规温育方法的OD值CV％分别为3．5,4．1、4．0、4．2。结论酶联免疫反应加速仪在确定最佳反应时间条件下，具有加速抗原抗体反应的作用，可以提高ELISA检测的灵敏度，为临床判读“灰区”标本的结果提供可靠依据，避免漏诊。

Objective To study the roles of enzyme-linked immunosorbent accelerometer played in detecting antibody to HCV by enzyme-linked immunosorbent assay （ELISA）. Methods 4 mixed serum of different S/CO were detected by enzyme-linked immunosorbent accelerometer and conventional incubation method ,while using real-time fluorescence quantitative polymerase chain reaction assay to detect HCV-RNA of gray zone specimens. Results There was significant difference （ P 〈 0.05 ） in anti-HCV OD values of 4 mixed serum by two methods. The qualitative results of 3 mixed serum were consistent. The result of another mixed serum which S/CO was 0.9 detected by conventional incubation method was negtive, but was positive detected by enzyme-linked immunosorbent accelerometer. Meanwhile, among 20 serum with S/CO of 0.9, HCV-RNA concentration of 14 serum was greater than 1 × 10^3 copies/mL. The CV% of OD values of 4 mixed serum by enzyme-linked immunosorbent accelerometer was 2.5,2.0,2.1,2.3, respectively, and the CV% by conventional incubation method was 3.5,4.1,4.0,4.2, respectively. 12onclusion When the best reaction time is determined, enzyme-linked immunosorbent accelerometer can accelerate the antifen-antibody reaction, and improve the sensitivity of ELISA. So it can provide a reliable basis for the clinical interpretation of specimens in gray areas to avoid misdiagnosis.