甲苯磺酸妥舒沙星荧光光谱法测定食品中痕量铝

Determination of Trace Aluminum in Food by Fluorescence Spectrometry with Tosufloxacin Tosylate

基于金属离子铝在pH 4.60 HAc-NaAc介质中,能与甲苯磺酸妥舒沙星(Tosufloxacin Tosylate,TFLX)形成稳定的络合物,且使甲苯磺酸妥舒沙星的荧光显著增敏,建立了测定金属离子铝的新方法。该体系的最大激发波长λex=365nm,最大发射波长λem=420nm。在1.4×10-8—5.6×10-6mol/L范围内铝离子的浓度与ΔF呈良好的线性关系,检出限为8.80×10-9mol/L,回收率在97.5%—101.2%之间,本法灵敏度较高,选择性好,结果令人满意。

In the medium of HAc-NaAc with pH of 4.60,aluminum metal ion combined with tosufloxacin tosylate（TFLX） to form a complex which greatly enhanced the fluorescence intensity of tosufloxacin tosylate,and a new method for determination of the content of aluminum was established.The maximum excitation wavelength λex of system was 365nm,and the maximum emission wavelength λem was 420nm.There was a good linear relationship between the concentration of aluminum ion and ΔF in the range of 1.4×10-8—5.6×10-6mol/L,and the minimum detection limit was 8.80×10-9mol/L with the recovery from 97.5% to 101.2%.The method is sensitive,selective with satisfying results.