大承气汤调控炎症介质在重症急性胰腺炎的表达意义

Significance Dachengqitang on regulation of inflammatory mediators in severe acute pancreatitis of Dachengqiatang

目的探讨大承气汤调控炎症介质在重症急性胰腺炎中表达意义。方法 30只健康Wistar大鼠随机分成3组,分别为:假手术组(A组)、重症急性胰腺炎模型组(B组)、重症急性胰腺炎+大承气汤治疗组(C组),每组10只。采用经胰胆管逆行注射5%牛磺胆酸钠法建立重症急性胰腺炎大鼠模型,于建立模型24小时后收集标本(静脉血、胰腺、肺及回肠组织),HE染色法显微镜下观察大鼠胰腺组织病理变化,ELISA法检测IL-6和IL-10表达,鲎试剂偶氮基质显色法检测内毒素(ET)表达。结果 A组大鼠胰腺仅有少量炎性细胞浸润,B组可见大量炎性细胞浸润、红细胞渗出、胰腺细胞水肿甚至坏死,C组大鼠病理改变明显减轻。B组血浆、肺及回肠组织中ET、IL-6、IL-10表达较A组明显升高(P<0.05),C组与B组相比,ET和IL-6明显降低,IL-10明显升高(P<0.05)。结论大承气汤通过调控炎症介质的表达在重症胰腺炎中起着重要的治疗作用,能够减轻病理损伤。

Objective To observe the significance of Dachengqitang expression in on regulation of inflammatory mediators of severe acute pancreatitis.Methods 30 Wistar rats were randomly divided into sham operation group（A group）,SAP group（B group）,SAP＋ulinastatin group（C group）.SAP was induced by retrograde injection of 5% sodium taurocholate.The pathological changes of pancreas was observed with microscope after HE staining.IL-6 and IL-10 was measure with ELISA.The endotoxin （ET） expression was detected with TAL Reagent chromogenic substrate.Results There were few inflammatory cell infiltrated in pancreas of A group and many inflammatory cells in that of B group.The levels of ET,IL-6 and IL-10 of lung,ileum and blood plasma in B group increased more significantly than that in A group（P〈0.05）.The level of ET and IL-6 decreased and IL-10 increased compared with that of A group and B group（P〈0.05）.Conclusion Dachengqitang could reduce the pathological damage by regulating the expression of inflammatory mediators in severe acute pancreatitis.