X线辐射对大鼠卵巢形态与功能的影响

The Effect of X-irradiation to the Morphology and Function of the Mice Ovary

目的:探讨X线电离辐射对大鼠卵巢形态与功能的影响。方法:实验分0,0.2,0.4,0.6,0.8,1.0Gy剂量组,每组各10只大鼠。采用HE染色观察各级卵泡数目,免疫组化法观察P53、Bcl-2蛋白表达,TUNEL法测定颗粒细胞中凋亡小体的表达。结果:随大鼠机体接受X线照射剂量增加,始基卵泡、初级卵泡、次级卵泡数目随之减少(P<0.05或P<0.01),以始基卵泡减少为著;不同剂量X线照射后窦状卵泡数目与假照射组差异无统计学意义(P>0.05)。当吸收剂量为0.2Gy,卵巢颗粒细胞中P53蛋白及凋亡小体表达较假照射组减少,Bcl-2蛋白有所增多(P<0.05或P<0.01),而吸收剂量在0.4～1.0Gy时,P53蛋白及凋亡小体的表达水平随吸收剂量的增加而上升(P<0.05或P<0.01),Bcl-2蛋白表达水平减少(P<0.01)。结论:X线可使大鼠卵巢组织中始基卵泡的数目有所减少,促进细胞发生凋亡,进一步影响颗粒细胞的内分泌功能。

Objective：To study the effect of X-irradiation to the morphology and function of the ovary of mice. Methods：The test is grouped by the dose：0,0.2,0.4,0.6,0.8,1.0 Gy. Use HE stain to observe the number of the every grade follicles,immunohistochemical technique to observe the expression of the P53 protein and Bcl-2 protein,TUNEL technique to observe the expression of the apoptotic bodies. Results：With increasing of the dose of X-ray ionizing radiation,the number of the primordial follicles,the primary follicles and the secondary follicles reduces（P0.01）,the most prominent is primordial follicles,but the antral follicles don’t change significantly（P0.05）. When the absorbed dose is 0.2 Gy,the expression of P53 protein and apoptotic bodies in ovarian granulosa cells is lower,however the expression of Bcl-2 protein levels is higher（P0.01）. But when absorbed dose is from 0.4 to 1.0 Gy,with increasing of dosage,the expression level of P53 protein and apoptotic bodies in ovarian granulosa cells increase,and the expression level of Bcl-2 protein deseases（P0.01）. Conclusions：X-irradiation can reduce the number of the primordial follicles,advance the apoptisis,then affect the regulation of gonadal hormone secretion.