microRNA在非小细胞肺癌组织中的表达及其临床应用研究

A study on expression of microRNA in tissue of non-small cell lung cancer and its clinical application

目的探讨microRNA(miRNA)在非小细胞肺癌(NSCLC)组织中的表达及其临床意义。方法用实时荧光定量PCR技术检测40例NSCLC患者癌组织与癌旁组织中miR-21、miR-20a、miR-31、miR-222、miR-223、miR-145、miR-126的表达变化。结果 NSCLC患者癌组织中miR-21、miR-20a、miR-31、miR-222较癌旁组织呈高表达(P<0.05),而miR-223、miR-145、miR-126呈低表达(P<0.05)。随着病情进展,miR-21、miR-222表达升高,miR-126表达下降。结论 miR-21、miR-20a、miR-31、miR-222、miR-145、miR-126、miR-223在NSCLC癌组织中表达存在差异,且miR-21、miR-222和miR-126与NSCLC病情的恶化发展相关,有助于预后判断。

Objective To detect and analyze the expression of microRNA（miRNA） in tissues of non-small cell lung cancer（NSCLS） by the real-time fluorescence quantitative PCR.Method The real-time fluorescence quantitative PCR was used to detect the expression of miR-21,miR-20a,miR-31,miR-222,miR-223,miR-145 and miR-126 in NSCLC tissues and the adjacent tissues from 40 cases.Results The expressions of miR-21,miR-20a,miR-31 and miR-222 were significantly higher in NSCLC tissues than those in adjacent tissues（P〈0.05）,and the expressions of miR-223,miR-145 and miR-126 were significantly lower in NSCLC cancer tissues than those in adjacent tissues（P〈0.05）.MiR-21 and miR-222 expressions increased with the progression of disease.The expression of miR-126 decreased with the progression of disease.Conclusion There were statistically significant differences of the expression of miR-21,miR-20a,miR-31,miR-222,miR-145 and miR-126 between NSCLC tissues and adjacent tissues.The expressions of miR-21,miR-222 and miR-126 were associated with the progression of disease development,so the detection showed very important diagnostic value and should be helpful for prognosis evaluation.