梅毒患者血清抗核抗体的检测及意义

目的研究梅毒螺旋体(Tp)感染后机体产生自身抗体的情况,探讨梅毒临床表现复杂多变的可能原因。方法以梅毒确证试验TPPA阳性的梅毒患者为研究对象,甲苯胺红不加热血清反应素试验(TRUST)检测患者非特异性Tp抗体,间接免疫荧光法检测患者血清中的ANA,x2检验统计ANA阳性率在梅毒患者中的显著性。结果梅毒患者血清ANA的阳性率(44.8%)与正常对照组(16.2%)相比,差异具有显著性,P<0.01;TRUST试验阳性的梅毒患者ANA阳性率(31.3%)分别高于TRUST试验阴性的梅毒患者(13.5%,P<0.05)和正常对照组(16.2%,P<0.01),差异均具有统计学意义,而TRSUT试验阴性的梅毒患者ANA阳性率与正常对照组比较差异无统计学意义,P=0.645。结论梅毒患者血清中自身抗体的产生几率显著高于正常人,梅毒患者临床表现复杂多变可能与机体自身抗体的产生有关。

Objective To research autoantibody generation condition in body after treponema pallidum infection,and to discuss the complex and changeable possible causes of syphilis in the clinical.Methods The research objects were syphilis patients who were confirmed by TPPA.Nonspecific Tp antibody and ANA were dedected in serum of syphilis patients with toluidine red unheated-serum test（TRUST） and indirect immunofluorescence test,.Significance of ANA positive rate in syphilis patients were statisticed with chi-square test.Results Compared with normal control group,the ANA positive rate（44.8%） in syphilis patients was significant difference,P0.01.the ANA positive rate（31.3%） in syphilis patients who were positive in TRUST was higher significantly than patients who were negative in TRUST（13.5%,P0.05） and normal control group（16.2%,P0.01）;But ANA positive rate was no statistically significant difference,compared normal control group,P=0.645.Conclusion Aotoantibodies produced significantly higher than normal control group in syphilis patients serum,the complex and changeable causes in the clinical maybe relevant with autoantibody generation of syphilis patients.