姜黄素预防造影剂对大鼠肾小管上皮细胞的损伤

Preventive effect of curcumin on contrast-induced nephropathy in rats

目的:探讨姜黄素对优维显370诱导的大鼠造影剂肾病(CIN)的预防作用。方法:雌性SD大鼠随机分为正常对照组(C)、造影剂肾病组(CIN)和姜黄素防治组(CUR),每组各5只。分别每隔15 min尾静脉注射吲哚美辛、L-NAME和优维显370(8 mL/kg)建立大鼠CIN模型。姜黄素在注射优维显370前5 d开始灌胃。所有大鼠均在注射造影剂后24h杀检。用TUNEL检测肾小管上皮细胞凋亡,硫代巴比妥酸法(TBA)测定肾脏丙二醛(MDA)水平。结果:姜黄素预防治疗显著降低造影剂肾病大鼠肾小管上皮细胞坏死指数、肾小管上皮细胞凋亡率、肾髓质淤血程度、肾脏MDA水平、血肌酐和肾重指数(均P<0.05)。结论:姜黄素可通过抗氧化活性而预防造影剂对大鼠肾小管上皮细胞损伤,从而预防CIN发病。

Objective To study the effect of curcumin on contrast-induced nephropathy(CIN) in rats. Methods Female Sprague-Dawley rats were randomly divided into three groups:control(C),iopromide-370 (CIN) and iopromide-370+curcumin(CUR).CIN was established by administering indomethacin,followed at 15 and 30 min,respectively,by L-NAME and iopromide-370(8 mL/kg) via caudal vein.Curcumin was administered 5 days prior to injecting of iopromide-370.All rats were killed at 24 hours after the administering of iopromide-370. Renal tubular cell apoptosis was evaluated by TUNEL.Kidney malonaldehyde(MDA) was detected by thiobarbituric acid test reaction(TBA).Results Pretreatment with curcumin significantly decreased tubular necrosis,medullary congestion,tubular apoptosis,kidney MDA,serum creatinine and kidney hypertrophy index of CIN.Conclusion This study demonstrated the protective role of curcumin against contrast-induced nephropathy in rats through its antioxidant effect.