应用光动力治疗幽门螺旋杆菌相关性胃炎的实验研究

The study of photodynamic therapy to Helicobacter pylori associated gastritis

目的:观察在不同照射强度下血卟啉介导的光动力疗法(PDT)治疗鼠幽门螺旋杆菌(Hp)相关性胃炎的疗效。方法:制备Hp相关性胃炎动物模型,分为A1和A2组,应用血卟啉衍生物(HpD)进行灌胃,C组(感染对照组),应用等量的生理盐水灌胃;3个感染组按顺序(A1,A2,C)均给予波长630nm输出功率分别为50、100、100 mW/cm^2经灌胃器照射,能量密度累积15 J/cm^2,非感染对照组(D组)未经PDT照射。照射后4周,处死所有小鼠,取胃窦和胃体部组织,行快速尿素酶试验和改良吉姆萨染色及H-E病理染色。结果:经PDT处理后,结合快速尿素酶试验和改良吉姆萨染色结果显示A2组Hp阴性率为73.33%(P<0.05)。H-E病理染色显示各感染组经PDT治疗后炎症明显减轻,非感染组经PDT治疗后可见轻微炎症反应。结论:PDT能明显减轻Hp相关性胃炎的炎症程度,并可杀灭Hp,不损伤正常胃黏膜。

Objective To evaluate empirical efficiency of the photodynamic therapy on mouse helicobacter pylori assiociated gastritis medicated by PDT in different intensity of the light. Methods Prepared animal models ： A1 ,A2 and C, 2 groups received HPD （A1,A2） and infected group C as control group, another group（D） was non-infected（n = 15 in each group）. Group C and D were given normal saline, The three groups（A1 ,A2,C） were illuminated with 630 nm light, respectively the power was 50,100,100 mW/cm^2, the power density is up to 15 J/ cm^2, all animals were killed after 4 weeks, the tissue of the body and antrum of stomach was taken . Fast urease test, improved Geimsa stain and H-E stain were performed. Results Geimsa showed the negative ratio of Hp reached 80% ,the negative ratio of group A2 was 73.33% combining the results of fast urease test with that of improved Geimsa stain, H-E stain suggested that inflamination of all infected groups were relieved after PDT, there were only mild inflamination in non-infected groups and no ulcer was found. Conclusion Gastritis associated with helicobacter pylori could be relieved and helicobacter pylori can be eradicated and normal gastric mucosa no damaged by PDT in proper intensity of light.