近红外光谱法快速测定山橿药材中球松素含量

Determination of Pinostrobin in Lindera Reflexa Helms by Near-infrared Spectroscopy Technique

目的利用近红外光谱(NIR)技术建立一种山橿中球松素含量的快速分析方法。方法以HPLC分析值为参照,采用近红外漫反射光谱技术采集66份山橿样品的近红外漫反射光谱,结合偏最小二乘法(PLS)建立了球松素含量的定量分析模型。结果所建模型的相关系数(R2)、外部验证均方差(RMSEP)和内部交叉验证均方差(RMSECV)分别为0.992 31、0.076 5和0.450 0;验证集样品的NIR测得值与药典法测得值进行配对T检验,差异无统计学意义。结论本方法操作简便、无污染、结果准确可靠,可用于山橿中球松素含量的快速测定。

OBJECTIVE To establish a rapid analytical method for pinostrobin in Lindera reflexa Helms by near-infrared（NIR） diffuse reflectance technique.METHODS Collecting NIR spectra by NIR diffuse reflectance spectroscopy with HPLC values anlysis as a reference to build calibration models by the partial least square（PLS）.RESULTS The correlation and coefficients of the PLS calibration models of NIR spectroscopy for phillyrin was 0.992 31,the root-mean-square error of prediction（RMSEP） was 0.076 5,the root-mean-square error of cross-validation（RMSECV） was 0.450 0;There was no statistical difference between NIR prediction of validation samples and HPLC measurements tested by paried sample T test.CONCLUSION The method is rapid,nondestructive and accurate,and can be applied to the fast determination of pinostrobin in extractum forsythiae siccus.