“张氏三伏贴”抗哮喘组方优化研究

Study on Optimized Formula of Zhang's Dog-days Paste for Treating Asthma

目的以均匀试验法筛选优化中药"张氏三伏贴"的处方组成。方法使豚鼠吸入雾化2%氯化乙酰胆碱和0.1%磷酸组胺生理盐水溶液诱发哮喘。以均匀试验设计对"张氏三伏贴"处方进行拆方,分析其抗哮喘的组方。结果本试验最终确定了"张氏三伏贴"对抗豚鼠急性诱发型哮喘的最佳处方组成为:麻黄、甘遂(生)、白芥子(炒)、细辛,其最佳剂量比为:200 mg.kg-1:75 mg.kg-1:100 mg.kg-1:150μL.kg-1。结论以此配伍组成的"张氏三伏贴"组方和阴性对照组相比较抗喘效果良好,安全性高。此组方的优化研究将为张氏三伏贴复方制剂开发提供依据。

OBJECTIVE To optimize the formula of Zhang＇s dog-days paste by uniform design.METHODS 2% acetylcholine chloride and 0.1% histamine phosphate in physiological saline solution were used to induce asthma in guinea pig.The uniform design was applied to decompose the Zhang＇s dog-days paste and evaluate its effect in treating asthma.RESULTS It was concluded that Ephedra,raw Kansui Root,fried Semen sinapis and Asarum oil were the effect components of Zhang＇s dog-days paste in treating asthma.The optimal dose ratio is 200 mg·kg-1：75 mg·kg-1：100 mg·kg-1：150 μL·kg-1,respectively.CONCLUSION The optimized prescription of Zhang＇s dog-days paste is effective and safe in treating asthma compared with negative control group.