铅和维生素C染毒对果蝇寿命和生殖力以及抗氧化系统的影响

Effects of Lead and Vitamin C on Fecundity,Life Span and Antioxidant System of Drosophila melanogaster

目的研究铅和维生素C(VC)染毒对野生型果蝇(Drosophila melanrogaster)寿命、生育力以及抗氧化系统的影响。方法将8 d内羽化且未交配的野生型红眼长翅果蝇随机分为10组,分别为对照(普通培养基)组和200、300、400、500 mg/L乙酸铅染毒组以及0、200、300、400、500 mg/L乙酸铅+150 mg/L VC染毒组,果蝇可自由摄食含上述物质的培养基。测定果蝇的寿命、子代数量及匀浆中SOD活力和MDA含量。结果随着铅染毒浓度的升高,果蝇体内MDA含量呈上升趋势,生殖力和体内SOD活力呈下降趋势,寿命逐渐缩短。添加VC后,果蝇体内MDA含量有所下降,生殖力和体内SOD活力有所上升,寿命有所延长。结论铅对果蝇的寿命和生殖力以及抗氧化系统有较大的损伤,VC可在一定程度上缓解铅离子对果蝇的毒害作用。

Objective To study the combined effect of lead and vitamin C （VC） on the life span,the fecundity and antioxidant system of the Drosophila melanrogaster. Methods The males and virgin females of Drosophila melanrogaster were divided randomly into ten groups and were fed with lead medium at different concentrations （0,200,300,400 and 500 mg/L）. And the Drosophila melanrogaster were also treated by 150 mg/L VC medium with the above concentrations of lead. The life span and the fecundity of Drosophila melanrogaster were recorded and the superoxide dismutase （SOD） activity and malondialdehyde （MDA） contents were determined. Results Compared with the control ,the life span was significantly （P〈0.05） shorter at 200 mg/L of lead. The number of first filial and SOD activity of the Drosophila melanrogaster decreased and MDA contents increased significantly （P〈0.05）. However, compared with those of corresponding lead treatment, at 200 mg/L of lead with 150 mg/L VC, the life span increased and was close to that in the control （P〉0.05）. At 300 mg/L of lead with 150 mg/L VC, the first filial of Drosophila melanrogaster increased and was close to that in the control （P〉0.05）,the increase of SOD activity and the decrease of MDA contents were still significant different from that in the control. These indicated VC had certain antagonistic effect on the toxicity of lead. Conclusion High lead exposure has an obvious adverse effect on the fecundity, the life span and antioxidant system in Drosophila melanrogaster and vitamin C can relieve the toxicity.