石家庄市集中空调系统水中嗜肺军团菌的脉冲场凝胶电泳指纹图谱分析

Pulsed-field Gel Electrophoresis Fingerprint Analysis of Legionella pneumophila in Water from Centralized Air Conditioning System in Shijiazhuang

目的对石家庄市集中空调系统水中分离的25株嗜肺军团菌进行脉冲场凝胶电泳（PFGE）分析，初步建立石家庄市嗜肺军团菌的PFGE分型数据库。方法采用PFGE技术，对2008年石家庄市集中空调系统水分离的嗜肺军团菌用AscI酶切，将PFGE图像用BioNumerics4．0软件处理后，采用统一的分子量标准对照沙门菌（BⅫnderup）H9812菌株进行校准后进行聚类分析。结果25株嗜肺军团菌的PFGE图谱共分为13种不同的PFGE带型，其中，LPA16．SJZ0006（7株）带型最多，占分离菌株的28．O％。聚类分析结果显示，所有血清1型的菌株间相似性系数为60％-100％；血清2—14型的菌株间相似性系数为80％～100％。结论本次从石家庄市集中空调系统水分离的嗜肺军团菌菌株呈多态性分布，基因组变异较大，同时也具有克隆化特征。

Objective To analyze the molecular types of Legionella（L.）pneumophila strains isolated from water in centralized air conditioning system （CACS） in Shijiazhuang and to develop the PulseNet-Shijiazhuang Database of L. pneumophila. Methods Pulsed field gel eleetrophoresis （PFGE） was used to analyze L.pneumophila collected from water in CACS of Shijiazhuang in 2008. After digesting with AscI , BioNumeries （Version 4） software was used to analyze the PFGE fingerprints. Cluster analysis was performed using the Marker H9812 calibration method. Results Twenty-five strains of L. pneumophila were divided into 13 banding patterns ,with SJZLP0006 （7 isolates） banding pattern strains accounted for 28% of bacterial strains. Cluster diagram showed all the serotype 1 strains similarity coefficients were 60%-100%; serum type 2-14 strains were 80%-100%. Conclusion L.pneumophila strains isolated from water in CACS in Shijiazhuang are with polymorphism in distribution and high genetic variations and also have the eharaeteristies of cloning.